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structural alleles of each other, and mutation d is a structural allele to a, b, and c, then d must contain a deletion of the bases mutated in a, b, and c. Once a sequence of deletion mutations covering the A and B cistrons was isolated, a minimal number of crosses was required to localize a new mutation to a portion of one of the cistrons. A second series of smaller deletions within each region was then isolated, further localizing the mutation ( g. 12.7). Next, each new mutant was crossed with each of the other mutants isolated in its subregion to localize the relative position of the new mutation. If the mutation was structurally allelic to a previously isolated mutation, it was scored as an independent isolation of the same mutation. If it was not a structural allele to any of the known mutations of the subregion, it was added as a new mutation point.The exact position of each new mutation within the region was

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where R is the tool nose radius, f is the feed and x is the distance between the nose top of the tool and the point of the transition as judged from the micrograph of the shoulder region (see Figure 345 c) For a typical example, such as silicon [111], using the foregoing equation for a tool having a nose radius of 08 mm and a rake angle of 25 , 05 m/rev feed and depth of cut of 55 m, it was found that tc = 57 nm In the ductile mode grinding process, each protruding abrasive grain on a grinding wheel generates an intense local stress field on contacting the workpiece surface According to Konig and Sinhoff s model [49], chip removal in ductile machining of an optical glass is caused by a hydrostatic shearing stress as a result of flattened dull edge grains (Figure 346) The shearing stress between glass lamellas causes frictional heat and plastification of the material, which finally results in a good surface quality Whereas sharp grains that exceed the maximum depth of cut (high in feeds) cause brittle fracture, and the work piece is damaged by deep cracks Zhong and Venkatesh [50] later modified this model by relating uneven protrusion heights of the grain with the critical depth concept to ductile streak formation Figure 347 shows the modified form of Konig s model The uneven protrusion height

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Thus, a high-Q lter has a narrow bandwidth, while a low-Q lter has a large bandwidth and is therefore less selective The quality factor of a lter provides an immediate indication of the nature of the lter The following examples illustrate the signi cance of these parameters in the response of various RLC lters

determined by the relative frequency of recombination between it and the known mutations of this region (see chapter 7). Benzer eventually isolated about 350 mutations from eighty different subregions de ned by deletion mutations. An abbreviated map is shown in gure 12.8. What conclusions did Benzer draw from his work First, he concluded that since all of the mutations in both rII cistrons can be ordered in a linear fashion, the original Watson-Crick model of DNA as a linear molecule was correct. Second, he concluded that reasonable inroads had been made toward saturating the map, localizing at least one mutation at every mutable site. Benzer reasoned that since many sites were represented by only one mutation, some sites must occur that were represented by zero mutations (i.e., not yet represented by a mutation). Since he had mapped about 350 sites, he calculated that there

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Compute the frequency response of the band-pass lter of Figure 619 for two sets of component values

Known Quantities:

Figure 12.7 Localization of an rII mutation by deletion mapping. Newly isolated mutants are crossed with mutants with selected deletions to localize the new mutation to a small region of the cistron. If the new mutant (e.g., r 960) is located in the A5c2a2 region, it would not produce the occasional wild-type by recombination with r1272, r1241, rJ3, r PT1, or rPB242 (the solid part of the bar indicates deleted segments). It would produce the wild-type by recombination with rA105 and r638, and thus the mutation would be localized to the A5 region. When crossed with r1605, r1589, and r PB230, it would produce only the rare recombinant with r PB230, indicating the mutation is in the A5c region. When crossed with r1993, r1695, and r1168, the mutant would produce the wildtype by recombination with r1993 and r1168, and the mutation would be localized to the A5c2a2 region. Finally, the mutant would be crossed pairwise with all the known mutants of this region to determine relative arrangement and distance. (Source:

Find: The frequency response, HV (j )

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