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where is a current ampli cation factor dependent on the physical properties of the transistor Typical values of range from 20 to 200 The operation of a pnp transistor is completely analogous to that of the npn device, with the roles of the charge carriers (and therefore the signs of the currents) reversed The symbol for a pnp transistor was shown in Figure 94 The exact operation of bipolar transistors can be explained by resorting to a detailed physical analysis of the npn or pnp structure of these devices The reader interested in such a discussion of transistors is referred to any one of a number of excellent books on semiconductor electronics The aim of this book, on the other hand, is to provide an introduction to the basic principles of transistor operation by means of simple linear circuit models based on the device i-v characteristic Although it is certainly useful for the non-electrical engineer to understand the basic principles of operation of electronic devices, it is unlikely that most readers will engage in the design of high-performance electronic circuits or will need a detailed understanding of the operation of each device The present chapter will therefore serve as a compendium of the basic ideas, enabling an engineer to read and understand electronic circuit diagrams and to specify the requirements of electronic instrumentation systems The focus of this section will be on the analysis of the i-v characteristic of the npn BJT, based on the circuit notation de ned in Figure 97 The device i-v characteristics will be presented qualitatively, without deriving the underlying equations, and will be utilized in constructing circuit models for the device The number of independent variables required to uniquely de ne the operation of the transistor may be determined by applying KVL and KCL to the circuit of Figure 97 Two voltages and two currents are suf cient to specify the operation of the device Note that, since the BJT is a three-terminal device, it will not be suf cient to deal with a single i-v characteristic; two such characteristics are required to explain the operation of this device One of these characteristics relates the base current, iB , to the base-emitter voltage, vBE ; the other relates the collector current, iC , to the collector-emitter voltage, vCE The latter characteristic actually consists of a family of curves To determine these i-v characteristics, consider the i-v curves of Figures 98 and 99, using the circuit notation of Figure 97 In Figure 98, the collector is open and the BE junction is shown to be very similar to a diode The ideal current source, IBB , injects a base current, which causes the junction to be forward-biased By varying IBB , one can obtain the open-collector BE junction i-v curve shown in the gure If a voltage source were now to be connected to the collector circuit, the voltage vCE and, therefore, the collector current, iC , could be varied, in addition to the base current, iB The resulting circuit is depicted in Figure 99(a) By varying both the base current and the collector-emitter voltage, one could then generate a plot of the device collector characteristic This is also shown in Figure 99(b) Note that this gure depicts not just a single iC -vCE curve, but an entire family, since for each value of the base current, iB , an iC -vCE curve can be generated Four regions are identi ed in the collector characteristic: 1 The cutoff region, where both junctions are reverse-biased, the base current is very small, and essentially no collector current ows 2 The active linear region, in which the transistor can act as a linear ampli er, where the BE junction is forward-biased and the CB junction is reverse-biased.

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Note: Plus sign indicates that female offspring are wild-type; minus sign indicates that they are mutant.

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(631)

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sites within a gene hampered the analysis of mutational sites within a gene by means of recombination If two mutant genes are functional alleles (involving different sites on the same gene), a distinct probability exists that we will get both mutant sites (and both wild-type sites) on the same chromosome by recombination (see g 124); but, in view of the very short distances within a gene, this probability is very low Although it certainly seemed desirable to map sites within the gene, the problem of nding an organism that would allow ne-structure analysis remained until Benzer decided to use phage T4 r II Screening Techniques Benzer used the T4 bacteriophage because of the growth potential of phages, in which a generation takes about an hour and the increase in numbers per generation is about a hundredfold.

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