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15.7 SOME RECENT RELEVANT WORK Nikoletseas et al. [21] propose extended versions of two data propagation protocols: the Sleep Awake Probabilistic Forwarding Protocol ( SW-PFR) and the Hierarchical Threshold-Sensitive Energy-Ef cient Network Protocol (HTEEN). These nontrivial extensions aim at improving the performance of the original protocols by (a) introducing sleep awake periods in the PFR protocol to save energy and (b) introducing a hierarchy of clustering in the TEEN protocol to better cope with large networks areas. The authors have implemented the two protocols and performed an extensive experimental comparison (using simulation) of various important measures of their performance with a focus on energy consumption. They investigate in detail the relative advantages and disadvantages of each protocol and discuss and explain their behavior. As a result of the above, they propose and discuss a possible hybrid combination of the two protocols toward optimizing certain goals. Recently, Boukerche et al. [22] proposed a novel and ef cient energy-aware distributed heuristic, which they refer to as EAD, to build a special rooted broadcast tree with many leaves that is used to facilitate data-centric routing in wireless microsensor networks. The EAD algorithm makes no assumption on local network topology and is based on residual power. It makes use of a neighboring broadcast scheduling and distributed competition among neighboring nodess. EAD basically computes a tree with many leaves. With the transceivers of all leaf nodes being turned off, the network lifetime can be greatly extended. Boukerche et al. [22] implement an EAD scheme and present an extensive simulation experiments to study the its performance. The experimental results indicate clearly that the EAD scheme outperforms previous schemes, such as LEACH among other protocols.

ACKNOWLEDGMENTS The work of Azzedine Boukerche has been supported by the Canada Research Chair Program, Canada Foundation Innovation Grant and Ontario Distinguished Researcher Award #201722. The work of Sotiris Nikoletseas has been supported by the IST/FET programme of the European Union under contract numbers IST-1999-14186 (ALCOM-FT), IST2001-33116 (FLAGS) and IST-2001-33135 (CRESCCO).

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86 The role of the glycoproteins GP1 and GP2 in the acid pH-dependent fusion determinant of arenavirus internalization was also demonstrated After exposure to acid pH, the LCMV spike glycoprotein complex constituted by GP1 and GP2 undergoes conformational changes characterized by alterations in the antibody binding ability of both glycoproteins and an irreversible dissociation of GP1 from the virions88,89 After a brief acid treatment, JUNV-infected cells expressing viral glycoproteins on their surface were able to induce the formation of syncytia by fusion with adjacent cells, providing evidence that conformational changes on the viral glycoproteins have occurred at low pH90 For LASV, a fusion activity is triggered by acid pH in a synthetic peptide homologous to an internal sequence of GP2.

DDmaRequest(TDmaChannel& aChannel, TCallback aCb=NULL, TAny* aCbArg=NULL, TInt aMaxTransferSize=0);

1. I. F. Akyildiz, W. Su, Y. Sankarasubramaniam, and E. Cayirci. Wireless sensor networks: A survey. Journal of Computer Networks, 38:393 422, 2002. 2. D. Estrin, R. Govindan, J. Heidemann, and S. Kumar. Next century challenges: Scalable coordination in sensor networks. In Proceedings of the 5th ACM/IEEE International Conference on Mobile Computing MOBICOM, 1999.

In constructing a request object, the driver must specify the channel with which it is to be used: aChannel It must also supply a callback function, aCb, which the channel DFC will call when the request completes (either following successful transfer or due to an error) The next parameter, aCbArg, is a driver-speci ed argument that will be saved by the framework and passed as an argument into the callback function Often a device driver will pass in a pointer to itself, allowing the callback function to access driver member functions when it is invoked The nal parameter, aMaxTransferSize, is used if the driver needs to specify the maximum fragment size to be applied during the transfer.

91 Altogether, these results allow one to assume that the interaction between GP1 and GP2 is altered under the acidic environment of the endosome, and, in consequence, a fusogenic peptide in GP2, which at neutral pH is located in a hidden position, becomes exposed and triggers the fusion between the viral envelope and the endosomal membrane Among probably early inhibitors of the replicative cycle of arenaviruses with therapeutic perspectives, one of the initial compounds studied more than three decades ago was amantadine, known as a blocker of in uenza virus uncoating Amantadine was an effective in vitro inhibitor of LCMV and several New World arenaviruses, but when it was assayed in vivo the results were discouraging because the administration of amantadine shortened the survival of guinea pigs and mice infected with JUNV and LCMV, respectively.

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